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Fig. 2 | Biology Direct

Fig. 2

From: p53 regulates expression of nuclear envelope components in cancer cells

Fig. 2

A fraction of p53-dependent genes binds to components of Nuclear Lamina and Nuclear Pore Complex. A ChIP-seq tracks of E2f4 in different cell lines (MEL, CH12, NIH3T3, C2C12); Lmnb1 and Tmpo belong to the Nuclear Lamina; Nup205, Nup107, Nup85, Nup35, belong to the Nuclear Pore Complex. B immunoblot and densitometry analysis showing Lamin B1 and p53 expression in KP CTR and KP shp53 cells; a representative micrograph of 2 independent experiments is shown. GAPDH is used as loading control. C immunofluorescence staining (left) and fluorescent tracing (right) of Lamin B1 in KP CTR and KP shp53 cells; nuclei are stained with DAPI, scale bar, 10 µm. D Lamin B1 fluorescence intensity quantification; A.U. is Arbitrary Units; data are presented as boxplot with Tukey’s whiskers; ****p-value < 0.0001. E heatmap of ATAC and RNAseq data of 5181 p53-dependent DNA regions; data are presented as shrunken log2 fold change (FC, shp53/shCTR). F–I chromosome enrichment analysis of p53-dependent (F and H), Lamin B1-bound (F) and NPC-bound genes (H). 502 genes are shared between p53 and Lamin B1 (G), and 547 are shared between p53 and NPC (I); data are presented as genes percentage (%) per chromosome; p-value is indicated by circles diameter and colour

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