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Fig. 8 | Biology Direct

Fig. 8

From: N6-methyladenosine-mediated SH3BP5-AS1 upregulation promotes GEM chemoresistance in pancreatic cancer by activating the Wnt signaling pathway

Fig. 8

SH3BP5-AS1 contributes to CTBP1 expression and increases tumor malignancy via the Wnt/β-catenin pathway. A–B Transwell and Sphere formation assays were performed in BxPC-3 cells after co-transfection with SH3BP5-AS1-shRNA and/or CTBP1 overexpression plasmid. C Stem cell-like cell markers Lin28, OCT4, NANOG, and SOX-2 were detected by western blot following the indicated transfections in BxPC-3 cells. D BxPC-3 cells with indicated transfections were treated with GEM at different concentrations for 48 h. Cell viability was measured by MTT assay. E BxPC-3 cells with indicated transfections were treated with GEM (1 μM) for 48 h. Cell viability was measured by MTT assay at different timepoints. F Correlation analysis of CTBP1 and miRNA-139-5p. G The proteins of Wnt/β-catenin pathway were detected by western blot following the indicated transfections in BxPC-3 cells. H Proteins of the Wnt/β-catenin pathway were detected by IHC following the indicated transfections in BxPC-3 cells. Data are shown as the mean ± SD. *P < 0.05; **P < 0.01

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