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Fig. 7 | Biology Direct

Fig. 7

From: N6-methyladenosine-mediated SH3BP5-AS1 upregulation promotes GEM chemoresistance in pancreatic cancer by activating the Wnt signaling pathway

Fig. 7

miR-139-5p sponging activity decreases CTBP1 levels, affecting the biological behavior of pancreatic cancer. A, B Transwell and Sphere formation assays were performed in BxPC-3 cells after co-transfection with miRNA-139-5p mimics and/or CTBP1 overexpression plasmid. C Stem cell-like cell markers Lin28, OCT4, NANOG, and SOX-2 were detected by western blot following the indicated transfections in BxPC-3 cells. D–E Transwell and Sphere formation assays were performed in MIA Paca-2 cells following the indicated transfections. F Stem cell-like cell markers Lin28, OCT4, NANOG, and SOX-2 were detected by western blot following the indicated transfections in MIA Paca-2 cells. G, H Transwell and Sphere formation assays were performed in PANC-1 cells after co-transfection with miRNA-139-5p-shRNA and CTBP1-shRNA. I Stem cell-like cell markers Lin28, OCT4, NANOG, and SOX-2 were detected by western blot following the indicated transfections in PANC-1 cells. J–L Pancreatic cancer cells with indicated transfections were treated with GEM at different concentrations for 48 h. Cell viability was measured by MTT assay. M–O Pancreatic cancer cells with indicated transfections were treated with GEM (1 μM) for 48 h. Cell viability was measured by MTT assay at different timepoints. Data are shown as the mean ± SD. *P < 0.05; **P < 0.01

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