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Fig. 3 | Biology Direct

Fig. 3

From: N6-methyladenosine-mediated SH3BP5-AS1 upregulation promotes GEM chemoresistance in pancreatic cancer by activating the Wnt signaling pathway

Fig. 3

Upregulation of SH3BP5-AS1 promotes pancreatic cancer cell resistance to gemcitabine in vitro and in vivo. A, B Colony formation in BxPC-3 cells transfected with shCtrl or shSH3BP5-AS1 and treated with DMSO or GEM. C BxPC-3 cells with stable silencing of SH3BP5-AS1 were treated with GEM at different concentrations for 48 h. Cell viability was measured by MTT assay. D Stable SH3BP5-AS1-silenced BxPC-3 cells were treated with GEM (1 μM) for 48 h. Cell viability was measured by MTT assay at different timepoints. E Representative images of the xenograft tumors of BxPC-3 cells with stable SH3BP5-AS1 silencing in nude mice following GEM or saline treatment. F, G Histograms displaying the tumor weight and volume. H Ki67 expression in BxPC-3 cells with stable SH3BP5-AS1 silencing in nude mice after GEM or saline treatment. I Table summarizing the results of lung metastasis. J Representative images of H&E staining of the xenograft tumors. Data are shown as the mean ± SD. *P < 0.05; **P < 0.01

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Biology Direct

ISSN: 1745-6150

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