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Fig. 2 | Biology Direct

Fig. 2

From: N6-methyladenosine-mediated SH3BP5-AS1 upregulation promotes GEM chemoresistance in pancreatic cancer by activating the Wnt signaling pathway

Fig. 2

Silencing of SH3BP5-AS1 expression inhibited the invasion, migration, and stemness of pancreatic cancer cells in vitro. vivo. A, B Transwell assay revealed the migration and invasion abilities of BxPC-3 and MIA Paca-2 cells transfected with shCtrl or shSH3BP5-AS1. C, D Histogram displaying the statistical results of the Transwell assay. E EMT-related markers were detected by western blot. F–I Sphere formation analysis. J–L Stem cell-like cell markers Lin28, OCT4, NANOG, and SOX-2 were detected by western blot and RT-qPCR. Data are shown as the mean ± SD of three replicates. *P < 0.05; **P < 0.01

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Biology Direct

ISSN: 1745-6150

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