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Fig. 2 | Biology Direct

Fig. 2

From: Expression of Synj2bp in mouse liver regulates the extent of wrappER-mitochondria contact to maintain hepatic lipid homeostasis

Fig. 2

Synj2bp silencing reduces the extent, but not the distance, of the wrappER-mitochondria contact. A Schematic representation of the protocol used in this study. B Immunoblot and densitometry analysis showing Synj2bp silencing in the livers of mice injected with AAV8-shSynj2bp. C Comparative quantitative proteomic analysis. The volcano plot shows the proteins that are down-and up-regulated in WAM-enriched fractions isolated from Synj2bp-silenced livers compared to control AAV8-shScrambled injected mice. D, E Electron microscopy morphometric analyses of the wrappER-mitochondria contact distance (D) and the percentage of mitochondria perimeter covered by the wrappER (E) in control and Synj2bp-silenced mouse livers. Note that MAMs (magenta) were excluded from these analyses. The Student’s t test was used to calculate p values. Data were obtained from mouse livers at 3 h postprandial unless otherwise indicated. WAM: wrappER-associated mitochondria

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Biology Direct

ISSN: 1745-6150

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