Fig. 4

LINC01419 recruits EZH2 to regulate the expression of FBP1. A The binding sites of EZH2 to FBP1 were predicted using the hTFtarget database; B FBP1 expression level in LUAD cells was measured by qRT-PCR; C Pearson correlation between LINC01419 and FBP1 was analyzed; D Pearson correlation between EZH2 and FBP1 was analyzed; E Schematic diagram of the FBP1 promoter region amplified by different primer sets in ChIP assays, Region2 and Region3 both contained a binding site, and Region 1 without binding site was used as a negative control; F ChIP assay was used to detect anti-EZH2 antibody as well as the EZH2 binding PCR products in Region2 and Region3; G sh-EZH2 or oe-EZH2 was constructed and co-transfected into LUAD cells with firefly luciferase vectors containing wild-type or mutant FBP1 promoters (FBP1 WT and FBP1 MT); pRL-SV40 was used as a control plasmid containing Renilla luciferase, and the ratio of firefly luciferase to Renilla luciferase was used to determine promoter activity in triplicate for each sample. H FBP1 mRNA expression level in LUAD cells transfected with sh-LINC01419 or oe-LINC01419; I FBP1 protein level in LUAD cells transfected with sh-LINC01419 or oe-LINC01419; J, K qRT-PCR and western blot experiments were performed to analyze the expression of FBP1 after silencing and overexpression of EZH2. *P < 0.05 is considered statistically significant