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Fig. 2 | Biology Direct

Fig. 2

From: Long-range enhancement of N501Y-endowed mouse infectivity of SARS-CoV-2 by the non-RBD mutations of Ins215KLRS and H655Y

Fig. 2

Ins215KLRS and H655Y enhance receptor binding and mouse infectivity by N501Y. A Schematic illustration of different mutants. B Representative images of syncytia formation upon expression of the indicated S glycoprotein in 293T-mACE2. Bar: 100 μm. C Quantification of syncytia formation upon expression of the indicated S glycoprotein in 293T-mACE2. Data are the mean ± SD of results from 4 fields (10× objective lens). D Expression of the luciferase reporter in 293T-mACE2 cells upon infection of viruses pseudotyped with indicated S glycoproteins. Data are the mean ± SD of triplicate measurements. E, F Immunoblot of the total cell lysate and immunoprecipitates (IP) derived from the 3*Flag tagged-mACE2 expressing cells transfected with SARS2-S WT or mutant plasmids. G quantification of S/ ACE2 was performed Data are the mean ± SD of triplicate measurements

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