Fig. 5
From: Phylogenomic analysis of the diversity of graspetides and proteins involved in their biosynthesis
Predicted precursors and loci organization in ATP-grasp branch 5. A Known precursor clusters versus new precursor clusters for branch 5. B Example of ATP-grasp subtree for Bacteroidetes with diverse precursors encoded in respective loci. The support value for the subtree is indicated. All cluster numbers identified in respective loci are indicated on the right. Manual alignment of most of the precursors in these loci is shown below. Protein local identifiers for the precursors are indicated on the left and the cluster numbers on the right. Amino acids highlighted as follows: conserved amino acids that could be involved in formation of ester or amide bonds are highlighted by red, GG-motifs are highlighted by blue, amino acids conserved in leader sequence are highlighted by green. C Example of diverse precursors in the locus of Aquimarina agarilytica. Locus organization is shown. Genes are shown by block arrows, roughly to scale. Genes are colored according to the homology and the key which is shown at the bottom of the Figure. Cluster number is indicated for all genes, except those coding for ATP-grasp proteins. Genbank accession and coordinates of the locus are indicated on the right. Manual alignment of the precursors is shown below. Local protein identifiers and respective cluster number are provided for each protein on the left and right respectively. Amino acids highlighted as follows: conserved amino acids that could be involved in formation of ester or amide bonds are highlighted by red, GG-motifs are highlighted by blue, amino acids conserved in leader sequence are highlighted by green. D Examples of selected loci from the branch 5. Selected loci organizations are shown. Genes are depicted by block arrows, roughly to scale. Genes are colored according to the homology and the key which is shown at the bottom of the Figure. Cluster number indicated above the arrows is provided for all genes, except those coding for ATP-grasp proteins. Genbank accession and coordinates of the locus and the name of the organism are indicated on the right of the locus schematics. Consensus sequences for two clusters precursors is shown below, cluster number are indicated on the left. Amino acids highlighted as follows: conserved amino acids that could be involved in formation of ester or amide bonds are highlighted by red, GG-motifs are highlighted by blue. Consensus sequences for LPL family is shown below the precursor sequences and the signature “LPL” motif is highlighted by purple