Skip to main content
Fig. 1 | Biology Direct

Fig. 1

From: Phylogenomic analysis of the diversity of graspetides and proteins involved in their biosynthesis

Fig. 1

Graspetide processing and comparison of the known graspetide BGCs with BGCs identified in this work. A General scheme of graspetide processing. Key features of the organization of graspetide biosynthetic gene clusters and subsequence processing of the precursor peptide are depicted. SPASM peptide maturase of the SPASM family, a radical SAM enzyme. B Comparison of ATP-grasp sequences identified in this work with ATP-grasp sequences reported by Iyer et al. [23] and Lee et al. [24]. ATP-grasp tree reconstructed in this work is schematically shown. Clades corresponding to experimentally characterized graspetides are marked on the right side of the tree. Two large subfamilies of ATP- (Actinobacterial and Bacteroidetes) mentioned in the work by Iyer et al. [23] are shown in gray. Yellow, ATP-grasp sequences that are at least 50% identical to and overlap by at least 80% of the protein length with those from Iyer et al. [23]. Green, ATP-grasp sequences that are at least 50% identical to and overlap by at least 80% of the protein length with those from Lee et al. [24] or from both studies. Black, the remaining ATP-grasp sequences that were not analyzed previously. Branches 1 to 6 selected for in depth characterization in this work are indicated on the right. Detailed information can be found in the Additional file 8: Table S1. C Correspondence between precursor sequences and ATP-grasp enzyme phylogeny. Known precursors are mapped on the same ATP-grasp tree if they are present in respective loci. Precursor cluster number corresponding to experimentally characterized graspetides and to Actinobacterial clade are indicated on the right by gray. Green—precursor similar to those reported in Lee et al. [24] paper or matching to one of the 12 motifs delineated in the same work (see "Methods" for detail). Yellow—precursors known either from Iyer et al. [23] work or annotated as such using respective PSSM (position specific score matrices) from CDD (conserved domain database). Black—ATP-grasp loci where known precursor peptides were not identified. Branches 7 and 8 selected for in depth characterization in this work are indicated on the right. Detailed information can be found in the Additional files 8, 10: Table S1 and S3. Ds Precursors identified in this work. Precursors identified in this work (red) are mapped on the same ATP-grasp tree if they are present in respective loci. Detailed information can be found in the Additional file 8: Table S1

Back to article page