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Fig.4 | Biology Direct

Fig.4

From: HIV-1LAI Nef blocks the development of hematopoietic stem/progenitor cells into myeloid-erythroid lineage cells

Fig.4

Effects of Nef on CD45 + CD14 + myeloid cells in the bone marrow, spleen and lymph nodes of humanized mice. LVX-vector or LVX-nef transduced CD34 + cells were transplanted into irradiated NCG mice. Three months after transplantation, mice were sacrificed and bone marrow, spleen and lymph nodes were harvested for CD45 + CD14 + cell analysis with flow cytometry. Representative flow images were shown in a, and the cumulative flow data of all the mice in each group were shown in b. The gating strategy was live > Zsgreen +  > CD45 + CD14 + . Spleens and lymph nodes were also paraffin embedded for H&E staining and IHC staining of CD45 + and CD14 + cells. Representative images of H&E and IHC staining from each group are shown in c (control group lymph node), d (control group spleen) and e (Nef group spleen), and the quantitative data of CD45 + and CD14 + cells of IHC staining in each group are shown in f (n = 6 for vector group and n = 7 for Nef group). *p < 0.05; **p < 0.01; ***p < 0.001

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