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Fig.3 | Biology Direct

Fig.3

From: HIV-1LAI Nef blocks the development of hematopoietic stem/progenitor cells into myeloid-erythroid lineage cells

Fig.3

Longitudinal effects of Nef on CD14 + myeloid and CD235a + erythroid cells in the peripheral blood of humanized mice. LVX-vector or LVX-nef transduced CD34 + cells were transplanted into irradiated NCG mice. Every month (for 3 months) after transplantation, mice were bled and peripheral blood was stained for CD45/CD235a and CD45/CD14 for flow analysis. The percentages of CD45 + CD14 + and CD45-CD235a + cells were compared between vector and nef group referring to myeloid and erythroid cells, respectively. Representative flow images of the blood cells are shown in b, and the cumulative flow data of three bleedings of all the mice in each group are shown in a (n = 6 for vector group and n = 7 for nef group). The gating strategy was live > CD45-CD235a + for erythroid and live > Zsgreen +  > CD45 + CD14 + for myeloid cells, respectively. **p < 0.01; *p < 0.05

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