Fig. 6

Salt-dependent up-regulation of the GG synthesis key enzyme, GG-phosphate synthase (GgpS). A Northern-blot showing accumulation kinetics of the ggpS transcript in response to salt shock of 4% [w/v] NaCl. Relative abundances were calculated after densitometric evaluation of the blot signals (signal obtained for 6 h was set as maximum, 100%). B Western blot confirming increased GgpS abundance in cells acclimated to 4% NaCl. Relative abundance was calculated after densitometric evaluation of blot signals (signal from control cells was set as 1). Data are the mean ± SD of values obtained from three individual blots. C Intracellular accumulation of GG in cells acclimated for 7 days to 4% NaCl. Data are the mean ± SD of 6 replicates