Fig. 5

EGFR-AS1 enhances CC cell growth via up-regulating ACTN4. a Transfection efficiency of pcDNA3.1/ACTN4 (labeled as ACTN4) in SiHa cells were examined by qRT-PCR. b, c The proliferation was analyzed by colony formation and EdU assays in SiHa cells transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + ACTN4. d, e The effect of sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + ACTN4 on cell apoptosis was evaluated by flow cytometry analysis and TUNEL assay. f, g Cell migration and invasion were estimated through western blot and transwell assays in SiHa cells with the transfection of sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + ACTN4. **P < 0.01. Scale bar: 100 µm