Fig. 4

ACTN4 is identified as the target of miR-2355-5p. a Three mRNAs with potential binding to miR-2355-5p were predicted by microT, miRmap, and RNA22 databases. b Expression levels of predicted mRNAs in CC cell lines and human normal cervical cell line was detected via qRT-PCR. c The enrichment of EGFR-AS1, miR-2355-5p and ACTN4 in RISC was certified by RIP assay. d The binding sites between ACTN4 (wild-type or mutant) and miR-2355-5p was manifested. e EGFR-AS1 expression was assessed after transfecting overexpression plasmid (labeled as EGFR-AS1) by qRT-PCR. f Luciferase activity of ACTN4-WT or ACTN4-MUT was estimated in CC cells transfected the indicated plasmids. g, h ACTN4 mRNA and protein levels in miR-2355-5p mimics or sh-EGFR-AS1#1 transfected cells were affirmed by qRT-PCR and western blot assays. **P < 0.01