Fig. 3

EGFR-AS1 functions as miR-2355-5p sponge. a, b Subcellular fractionation and FISH assays were conducted for detecting the distribution of EGFR-AS1 in CC cells (Scale bar: 10 µm). c DIANA tool was applied to predict candidate miRNAs that could bind to EGFR-AS1. d RNA pull down assay was employed to screen out the potential miRNAs for EGFR-AS1 in CC cells. e MiR-2355-5p expression was measured in CC cell lines and human normal cervical cell line by qRT-PCR. f RIP assay was used to identify the enrichment of EGFR-AS1 and miR-2355-5p in RNA-induced silencing complex (RISC). g The binding sites between EGFR-AS1 (wild-type or mutant) and miR-2355-5p were displayed. h CC cells were transfected with miR-2355-5p mimics and the transfection efficiency was identified by qRT-PCR. i Luciferase activity of EGFR-AS1-WT or EGFR-AS1-MUT was evaluated in CC cells with the transfection of miR-2355-5p mimics or NC mimics. **P < 0.01