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Fig. 2 | Biology Direct

Fig. 2

From: H3K27ac-activated EGFR-AS1 promotes cell growth in cervical cancer through ACTN4-mediated WNT pathway

Fig. 2

EGFR-AS1 is induced by CBP-mediated H3K27ac in CC cells. a The enrichment of H3K27ac at EGFR-AS1 promoter region and the amplified region were predicted by UCSC database. b H3K27ac level in SiHa, CaSki and Ect1/E6E7 cells at EGFR-AS1 promoter region was confirmed by ChIP assay. c EGFR-AS1 expression in CC cells transfected with C646 or DMSO was detected by qRT-PCR. d CBP expression in CC cell lines and human normal cervical cell line was detected by qRT-PCR. e CBP mRNA and protein levels were evaluated in sh-CBP transfected cells through qRT-PCR and western blot assays. f qRT-PCR was utilized for evaluating the effect of CBP depletion on EGFR-AS1 expression. g ChIP assay was performed to assess CBP enrichment at EGFR-AS1 promoter region. h The binding of H3K27ac to EGFR-AS1 promoter in sh-CBP group or sh-NC group was determined by ChIP assay. *P < 0.05; **P < 0.01

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