Fig. 5

LINC00885 upregulated BAZ2A through regulating miR-3150b-3p. A The putative target mRNAs (BAZ2A, ZDHHC9, PDE4A, RBFOX2, AHDC1, and NAV1) for miR-3150b-3p were predicted on RNA22, miRmap, microT, and PicTar databases. B RT-qPCR was done for detecting the expression level of BAZ2A in CC cell lines and cervical epithelial immortalized cell line (H8). C RT-qPCR was applied for the measurement of BAZ2A expression in SiHa and HeLa cells under the influence of LINC00885 overexpression or knockdown. D RT-qPCR analysis was done for the detection of BAZ2A expression in SiHa cells with miR-3150b-3p inhibition and in HeLa cells with miR-3150b-3p overexpression. E The binding sequences of miR-3150b-3p on BAZ2A 3’UTR were demonstrated. F, G Luciferase reporter and RIP assays validated the interaction between miR-3150b-3p and BZA2A. H RT-qPCR was applied for the measurement of miR-3150b-3p and BAZ2A in adjacent non-tumor and CC tumor tissues. I Spearman’s correlation analysis showed the negative correlation between miR-3150b-3p and LINC00885 or BAZ2A, and the positive correlation between LINC00885 and BAZ2A in CC tissues. *P < 0.05, **P < 0.01, ***P < 0.001, n.s.: no significance