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Fig. 6 | Biology Direct

Fig. 6

From: Rab32 uses its effector reticulon 3L to trigger autophagic degradation of mitochondria-associated membrane (MAM) proteins

Fig. 6

Rab32 cooperates with the RTN3 long form. A Immunoblot and densitometry analysis of control pcDNA3 and Q85L-FLAG transfected cells indicates only RTN3L levels decrease with Q85L, while RTN3S, FAM134B, Sec62, and CCPG1 do not show changes. n = 3; **p ≤ 0.01. B Co-immunoprecipitation of Rab32 constructs with endogenous RTN3S. MCF7 cells were transfected for 48 h with FLAG-tagged Rab32 constructs, followed by crosslinking, lysis and incubation with anti-FLAG antibodies. Immunoprecipitates were analyzed for anti-FLAG and co-immunoprecipitating endogenous short reticulon-3 (RTN3S). C Immunoblot as in A with 100 nM Bafilomycin A1 incubation for 48 h shows accumulation of RTN3L and p62 with Q85L. D Immunoblot of MCF7 cells transfected with pcDNA3 and Rab32Q85L co-transfected with control or siRNA against RTN3. RTN3L and FLAG gels assay for the amounts of RTN3L and Rab32. TMX1 amounts were used to assay for autophagy of MAM proteins

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