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Fig. 6 | Biology Direct

Fig. 6

From: LncRNA MIR205HG accelerates cell proliferation, migration and invasion in hepatoblastoma through the activation of MAPK signaling pathway and PI3K/AKT signaling pathway

Fig. 6

MIR205HG sponges miR-205-5p to activate PI3K/AKT signaling pathway. A MiR-205-5p expression was examined after MIR205HG was knocked down. B RNA pull down assay detected the accumulation of MIR205HG in the biotin-labeled miR-205-5p-Wt probe and biotin-labeled miR-205-5p-Mut probe. C Transfection of miR-205-5p mimics to overexpress miR-205-5p. D The luciferase activities in the MIR205HG-Wt group and MIR205HG-Mut group were detected when miR-205-5p was overexpressed. E The protein levels of related factors in the PI3K/AKT signaling pathway were analyzed by western blot when miR-205-5p was up-regulated. GAPDH and U6 were used as internal references for RT-qPCR. Each experiment was performed in triplicate. Student’s t test, one-way ANOVA and two-way ANOVA were adopted for statistics. **P < 0.01

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