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Fig. 4 | Biology Direct

Fig. 4

From: LncRNA MIR205HG accelerates cell proliferation, migration and invasion in hepatoblastoma through the activation of MAPK signaling pathway and PI3K/AKT signaling pathway

Fig. 4

MIR205HG modulates MAPK expression and activates MAPK signaling pathway by competitively binding to miR-514a-5p. A The luciferase activities of common signaling pathways were tested when MIR205HG was down-regulated. B The protein levels of related factors in the MAPK signaling pathways were analyzed by western blot. The images of original blots were shown in Supplementary File. C Binding sites between miR-514a-5p and MAPK9. D RIP assay exhibited the relationship among MIR205HG, miR-514a-5p and MAPK9. E The interaction between miR-514a-5p and MAPK9 was confirmed by luciferase reporter assay. F Transfection of miR-514a-5p inhibitor to lessen miR-514a-5p expression. G–H MAPK9 expression was detected in HB cells in different groups. GAPDH and U6 were used as internal references for RT-qPCR. Each experiment was performed in triplicate. Student’s t-test, one-way ANOVA and two-way ANOVA were adopted for statistics. **P < 0.01

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Biology Direct

ISSN: 1745-6150

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