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Fig. 3 | Biology Direct

Fig. 3

From: LncRNA MIR205HG accelerates cell proliferation, migration and invasion in hepatoblastoma through the activation of MAPK signaling pathway and PI3K/AKT signaling pathway

Fig. 3

MIR205HG binds to miR-514a-5p in HB cells. A RIP assay exhibited the affinity of MIR205HG with Ago2 protein. B MiR-514a-5p expression in HB cells was examined by RT-qPCR. C RNA pull down assay verified the relationship between MIR205HG and miR-514a-5p. D Binding regions between MIR205HG and miR-514a-5p. E MiR-514a-5p expression was enhanced by transfection of miR-514a-5p mimics. F Luciferase reporter assay examined the luciferase activity in MIR205HG-Wt group and MIR205HG-Mut group after transfection of miR-514a-5p mimics. GAPDH and U6 were used as internal references for RT-qPCR. Each experiment was performed in triplicate. Student’s t test, one-way ANOVA and two-way ANOVA were adopted for statistics. *P < 0.05, **P < 0.01

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