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Fig. 2 | Biology Direct

Fig. 2

From: Circ_0067680 expedites the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells through miR-4429/CTNNB1/Wnt/β-catenin pathway

Fig. 2

Circ_0067680 knockdown hinders the osteogenic differentiation of hBMSCs. A Circ_0067680 was knocked down in hBMSCs and was verified by RT-qPCR. B CCK-8 assay detected the cell viability in hBMSCs after circ_0067680 silence. C ALP activity from ALP staining assay when circ_0067680 was down-regulated. D, E The expression of RUNX2, OSX and OCN was analyzed by RT-qPCR and western blot analyses after circ_0067680 was silenced. F Flow cytometry was used to detect the condition of cell apoptosis when transfected with sh-circ_0067680#1/2. **P < 0.01

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