Skip to main content
Fig. 4 | Biology Direct

Fig. 4

From: LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4

Fig. 4

NORAD accelerated OSCC progression by targeting miR-577/TPM4. a The overexpression efficiency of pcDNA3.1/TPM4 in OSCC cells and TPM4 expression in cells transfected with sh-NORAD#1 were examined by RT-qPCR assays. bd The proliferation of cells co-transfected with sh-NORAD#1 and pcDNA3.1/TPM4 was detected by CCK-8, colony formation and EdU assays. e The apoptosis rate of cells co-transfected with sh-NORAD#1 and pcDNA3.1/TPM4 was assessed by flow cytometry analysis. f Western blot assay was carried out to measure the protein level of apoptosis-associated proteins in cells co-transfected with sh-NORAD#1 and pcDNA3.1/TPM4. g Transwell assays were carried out to estimate the migration ability of cells co-transfected with sh-NORAD#1 and pcDNA3.1/TPM4. (H) Western blot assay was utilized to measure the protein level of EMT-associated proteins. **P < 0.01

Back to article page