Fig. 3

TPM4 was the downstream target of miR-577. a StarBase database was utilized to predict the potential mRNAs which could bind to miR-577. b RNA pull down assays were carried out to prove whether miR-577 could bind to TPM4. c Bioinformatics analysis was adopted to predict the binding sites between miR-577 and TPM4. d The expression of TPM4 was tested in OSCC cell lines. e RIP assay was used to confirm whether miR-577 and TPM4 coexisted in RISCs. f Luciferase reporter assays were performed to verify whether miR-577 could bind to TPM4. g The expression and protein level of TPM4 in cells transfected with miR-577 mimics was examined by RT-qPCR and western blot assays. **P < 0.01