Fig. 2

NORAD bound to miR-577 in OSCC. a FISH assays were conducted to evaluate the distribution of NORAD in nucleus and cytoplasm. b Possible miRNAs which could bind to NORAD were predicted by starBase database. c RT-qPCR assays were used to examine the expression of miR-577 and miR-642-5p in OSCC cell lines d Bioinformatics analysis was adopted to predict the potential binding sites between miR-577 and NORAD. e RNA pull down assay was performed to verify whether miR-577 could bind to NORAD. f RT-qPCR assay was performed to detect the overexpression efficiency of miR-577 mimics. g Luciferase reporter assays were carried out to verify whether miR-577 could bind to NORAD. h The expression of miR-577 in HSC-4 and UM1 cells transfected with sh-NORAD#1 was estimated by RT-qPCR assay. **P < 0.01