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Fig. 1 | Biology Direct

Fig. 1

From: Long non-coding RNA SNHG8 enhances triple-negative breast cancer cell proliferation and migration by regulating the miR-335-5p/PYGO2 axis

Fig. 1

SNHG8 facilitates the proliferation, migration and EMT of TNBC cells. A The expression of SNHG8 was evaluated in four types of TNBC cells (MDA-MB-231, MDA-MB-436, BT-549 and HCC1937) and in normal breast epithelial cells (MCF-10A) by RT-qPCR assay. B RT-qPCR analysis indicated that SNHG8 expression was decreased in MDA-MB-231 and BT-549 cells respectively transfected with 3 shRNAs targeting SNHG8. C, D The influence of SNHG8 silencing on the proliferation of TNBC cells was examined using colony formation and EdU assays. E We detected the migratory capacity of TNBC cells affected by SNHG8 knockdown through Transwell assay. F RT-qPCR assay and western blot assay were adopted to analyze the E-cadherin, N-cadherin, Vimentin, MMP2 and MMP7 mRNA and protein levels with or without SNHG8 silencing. *P < 0.05, **P < 0.01

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Biology Direct

ISSN: 1745-6150

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