Skip to main content
Fig. 5 | Biology Direct

Fig. 5

From: High-fat diet impairs ferroptosis and promotes cancer invasiveness via downregulating tumor suppressor ACSL4 in lung adenocarcinoma

Fig. 5

High-fat treatment promoted cell survival, invasion, and migration and inhibited ferroptosis. a The effect of palmitic acid treatment on ACSL4 expression in 4 different lung adenocarcinoma cell lines. Representative Western blot results were shown and ACTIN was used as the loading control. The relative blot intensity was listed below each band. b Cell viability was analyzed by MTT in 4 different lung adenocarcinoma cell lines treated with or without palmitic acid. c Cell migration and invasion were analyzed by the transwell assays in lung adenocarcinoma cells treated with or without palmitic acid. d Cell migration was analyzed by wound healing assay in lung adenocarcinoma cells treated with or without palmitic acid. e Liperfluo-based lipid peroxidation assay was used to monitor the ferroptosis levels in lung adenocarcinoma cells treated with erastin and/or palmitic acid

Back to article page