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Table 1 HHPred search with the N-terminal part of the four human TMTCs against PDB (PDB_mmCIF70_29_May, version 29/05/2020)

From: Conserved sequence motifs in human TMTC1, TMTC2, TMTC3, and TMTC4, new O-mannosyltransferases from the GT-C/PMT clan, are rationalized as ligand binding sites

PDB ID

N-TMTC1

(1–456)

N-TMTC2

(1–475)

N-TMTC3

(1–426)

N-TMTC4

(1–462)

5ezmA/5f15A

(578 AA)

[58]

1.9E-22

5.9E-19

1.3E-21

4.2E-21

Q: 1–456

Q: 1–475

Q: 2–424

Q: 1–460

T: 7–399

T: 33–400

T: 27–395

T: 12–395

6s7tA

(826 AA)

[59]

1.8E-17

2.3E-15

3.1E-17

1.2E-16

Q: 1–456

Q: 2–475

Q: 2–425

Q: 1–462

T: 48–560

T: 75–559

T: 70–558

T: 53–559

6s7oA

(705 AA)

[65]

2.2E-17

4.9E-15

3.4E-17

4.1E-17

Q: 8–456

Q: 2–475

Q: 1–426

Q: 1–461

T: 1–479

T: 21–480

T: 12–477

T: 1–476

6eznF

(718 AA)

[74]

1.4E-17

3E-15

5.1E-17

1.7E-16

Q: 9–454

Q: 1–474

Q: 2–424

Q: 3–461

T: 1–467

T: 19–469

T: 14–465

T: 1–466

3wajA

(875 AA)

[75]

2.6E-17

8.6E-15

1.8E-17

1E-15

Q: 12–456

Q: 1–474

Q: 1–425

Q: 7–459

T: 1–490

T: 16–490

T: 9–489

T: 1–486

5oglA

(713 AA)

[60]

2E-16

5.7E-14

4.5E-16

1.1E-15

Q: 11–455

Q: 2–475

Q: 1–426

Q: 4–462

T: 1–432

T: 18–343

T: 12–433

T: 1–434

6p25A/6p2rA

(817 AA)

[59]

5.7E-14

7.8E-13

6.3E-14

1.8E-13

Q: 1–453

Q: 2–473

Q: 1–257

Q: 1–303

T: 26–703

T: 53–705

T: 45–290

T: 32–304

7bvfA

(1102 AA)

[76]

1.9e-12

1.2e-10

1.1e-12

2.2e-11

Q: 33–456

Q: 7–473

Q: 13–426

Q: 26–462

T: 263–631

T: 263–631

T: 263–633

T: 263–630

6sniX/6snhX

(562 AA)

[77]

1.5E-10

1.3E-08

1.8E-11

1.2E-09

Q: 1–416

Q: 1–434

Q: 1–388

Q: 1–424

T: 30–411

T: 54–411

T: 49–411

T: 35–411

  1. The eight essentially full-length hits with best E-values and sequence coverage > 90% are tabulated: 5ezm, crystal structure of ArnT from Cupriavidus metallidurans in the apo state [58], 5f15 is the same as 5ezm but with undecaprenyl phosphate as analogue for a lipid-linked sugar substrate; 6s7t, cryo-EM structure of human oligosaccharyltransferase complex OST-B [59]; 6s7o, cryo-EM structure of human oligosaccharyltransferase complex OST-A [65]; 6ezn, cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex [74]; 3waj, crystal structure of the Archaeoglobus fulgidus oligosaccharyltransferase (O29867_ARCFU) complex with Zn and sulfate [75]; 5ogl, structure of bacterial oligosaccharyltransferase PglB in complex with an acceptor peptide and an lipid-linked oligosaccharide analogue [60]; 6p25/6p2r, structure of Saccharomyces cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor/without peptide ligand [59]; 7bvf_A, Cryo-EM structure of Mycobacterium tuberculosis arabinosyltransferase EmbA-EmbB-AcpM2 in complex with ethambutol [76]. We added also 6sni/6snh (cryo-EM structure of nanodisc reconstituted yeast ALG6 in complex with 6AG9 Fab or with Dol25-P-Glc [77]) because of the much shorter template length. For each query and each PDB structure (listed as PDB ID), we provide the E-value and the sequence ranges hit in the query (Q) and in the template (T; we also provide the length of the template in parentheses below the PDB identifier). The uppercase letter behind the PDB identifier denotes the relevant chain