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Fig. 1 | Biology Direct

Fig. 1

From: Conserved sequence motifs in human TMTC1, TMTC2, TMTC3, and TMTC4, new O-mannosyltransferases from the GT-C/PMT clan, are rationalized as ligand binding sites

Fig. 1

Grand alignment of N-terminal segments of TMTCs together with sequences of selected sugar transferases with known 3D structure. We show the grand alignment of the full set of the N-terminal segments of TMTCs from six organisms (Homo sapiens (Hs), Bos taurus (Bt), Gallus gallus (Gg), Xenopus laevis (Xl), Danio rerio (Dr), Drosophila melanogaster (Dm)) together with the sequences taken from 5ezm chain A [58], 6s7t chain A [59], 5ogl chain A [60] and 6p25 chain A [59]. For supporting navigation in the alignment, the location of the TMs in human TMTC1 and in 5ezm are shown. The figure was generated with Jalview [61] using an externally created and manually edited multiple alignment (in the SEAVIEW environment [62, 63]). The location of TMs in TMTC1 follows the observations from the 3D structural model created in the course of this work and, at some places, does differ slightly from the sequence-analytic predictions provided in Additional File 2. The following sequence segments have been excluded from the alignment and replaced by “XX”: in TMTC1_B, 244–304 after TM6; in TMTC1_Gg, 251–310 after TM6; in TMTC1_Dm, 358-417after TM8; in TMTC2_Hs, 337–393 after TM8; in TMTC2_Bt (G3MY32_BOVIN), 334–393 after TM8; in TMTC2_Gg (F1NPM4_CHICK), 324–380 after TM8; in TMTC2_Xl, 337–393 after TM8; in TMTC2_Dr (F1R0Y9_DANRE), 346–401 after TM8, in TMTC2_Dm, 360–504 after TM8; in 6S7T, 288–348 after TM6 and 486–535 after TM10; in 6P25, 219–261 after TM6, 312–531 after TM7 and 560–585. Please note that, as result of the excluded sequence stretches in some sequences, the residue numbering in the figure might deviate from the residue numbering in the respective entry of the sequence database. Additional information for this figure is provided in Additional Files 1 and 2 available with this article. For locating specific residues in the alignment, we recommend first finding the nearby TMs and then looking for conserved motifs next to them

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