Fig. 6
Detection of the BMP signaling pathway target genes in the Sox2-Cre;Fam20cfl/fl mice. A and B, IHC analyses (signal in brown) of P21 on the sagittal sections of the mandibular incisors of 3-day-old Fam20cfl/fl (A) and Sox2-Cre;Fam20cfl/fl mice (B). a1 and b1 are the higher magnification views of the presecretory ameloblasts in black boxes in A and B, respectively. P21 was reduced significantly in the incisors of Sox2-Cre;Fam20cfl/fl mouse compared to the Fam20cfl/fl mouse. C, Western-immunoblotting analyses of P21 in the enamel organ cells of 3-day-old Fam20cfl/fl and Sox2-Cre;Fam20cfl/fl mouse incisors. D, Relative protein levels of P21 determined by Western immunoblotting (n = 3; * = p < 0.05). The protein level of P21 was significantly decreased in the enamel organ cells of the Sox2-Cre;Fam20cfl/fl mice compared to the Fam20cfl/fl mice. E and F, IHC analysis of MSX2 on the sagittal sections of the mandibular incisors of 3-day-old Fam20cfl/fl and Sox2-Cre;Fam20cfl/fl mice. e1 and f1 are the higher magnification views of the secretory ameloblasts in black boxes in E and F, respectively. The Sox2-Cre;Fam20cfl/fl mice had reduced nuclear localization of MSX2 in ameloblasts compared to that of the Fam20cfl/fl mice. G and H, ISH analyses (signal in purple) of Msx2 mRNA on the sagittal sections of the mandibular incisors of newborn Fam20cfl/fl and Sox2-Cre;Fam20cfl/fl mice. g1 and h1 are the higher magnification views of the secretory ameloblasts in black boxes in G and H, respectively. The mRNA level of Msx2 was reduced in the ameloblasts of Sox2-Cre;Fam20cfl/fl mice, compared to the control mice. I and J, IHC analysis of LAMA3 on the sagittal sections of the mandibular incisors of 3-day-old Fam20cfl/fl and Sox2-Cre;Fam20cfl/fl mice. i1 and j1 are the higher magnification views of the secretory ameloblasts in black boxes in I and J, respectively. The level of LAMA3 was markedly reduced in the Sox2-Cre;Fam20cfl/fl mice compared to the control mice. Scale bars: 500 μm in A, B, E, F, G, H, I and J; 20 μm in a1, b1, e1, f1, i1 and j1; 10 μm in g1 and h1