Fig. 3
Reduced expressions of the secretory ameloblast markers in the Sox2-Cre;Fam20Cfl/fl mice. A-F, ISH analyses (signal in purple) of AMEL (A and B), AMBN (C and D) and ENAM (E and F) on the sagittal sections of the mandibular incisors of 3-day-old Fam20cfl/fl (A, C and E) and Sox2-Cre;Fam20cfl/fl (B, D and F) mice. a1, b1, c1, d1, e1 and f1 are the higher magnification views of the secretory ameloblasts in black boxes in A, B, C, D, E and F, respectively. The expressions of AMEL, AMBN and ENAM were sharply decreased in the incisors of Sox2-Cre;Fam20cfl/fl mouse compared to the Fam20cfl/fl mouse. IHC analyses of AMEL (G and H) and AMBN (I and J) on the sagittal sections of the mandibular incisors of 3-day-old Fam20cfl/fl (G and I) and Sox2-Cre;Fam20cfl/fl (H and J) mice. g1, h1, i1 and j1 are the higher magnification views of the secretory ameloblasts in black boxes in G, H, I and J, respectively. Both the protein level of AMEL and AMBN were markedly reduced in the incisors of Sox2-Cre;Fam20cfl/fl mouse compared to the Fam20cfl/fl mouse. Scale bars: 500 μm in A - J; 50 μm in a1, b1, c1, d1, e1, f1; 20 μm in g1, h1, i1, j1