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Fig. 5 | Biology Direct

Fig. 5

From: Long non-coding RNA LINC00426 contributes to doxorubicin resistance by sponging miR-4319 in osteosarcoma

Fig. 5

LINC00426 promoted OS doxorubicin resistance by sponging miR-4319. a-f MG63/DXR and KHOS/DXR cells were co-transfected with si-NC + NC-inhibitor, si-LINC00426–1 + NC-inhibitor or si-LINC00426–1 + miR-4319-inhibitor. a The RNA levels of miR-4319 in these co-transfected MG63/DXR and KHOS/DXR cells were determined by qRT-PCR. b and c Cell counting kit-8 assays were performed to examine the cell proliferation rate of these co-transfected MG63/DXR and KHOS/DXR cells in response to doxorubicin. d Clone formation assays were performed to examine cell vitality of these co-transfected MG63/DXR and KHOS/DXR cells to doxorubicin exposure (10 μg/ml, 14 days). e IC50 values of these co-transfected MG63/DXR and KHOS/DXR cells. f Caspase-3 activity assays were performed to examine cell apoptosis of these co-transfected MG63/DXR and KHOS/DXR cells to doxorubicin exposure (0.1 μg/ml, 48 h). The data represent the mean ± SD. Two-way ANOVA for B and C, Student’s t-tests for others. *P < 0.05; **P < 0.01; ***P < 0.001

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