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Fig. 5 | Biology Direct

Fig. 5

From: MDA19, a novel CB2 agonist, inhibits hepatocellular carcinoma partly through inactivation of AKT signaling pathway

Fig. 5

CB2 knockdown inhibited cell apoptosis of HCC. NC: HCC cells were transfected with siNC (50nM) and incubated for 48h; CB2-KD: HCC cells were transfected with CB2 siRNA (50nM) and incubated for 48 h; MDA19 + CB2-KD: HCC cells were transfected with CB2 siRNA (50nM) and treated with MDA19 (30 μM for Hep3B and 40μM for HepG2) for 48 h. a Cell apoptosis of HCC cells was detected by a PI-AnnexinV-FITC assay and flow cytometry; The data were analyzed using FlowJo software.; (b) The expression of apoptosis related proteins Bcl2 and Caspase3 was detected by western blot and analyzed by Image J software. All experiments were performed at 3 times. *P < 0.05

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