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Fig. 4 | Biology Direct

Fig. 4

From: MEGAN-LR: new algorithms allow accurate binning and easy interactive exploration of metagenomic long reads and contigs

Fig. 4

The LAST+MEGAN-LR pipeline. Long reads or contigs are aligned against the NCBI-nr database using LAST and the resulting MAF file (multiple alignment format) is converted to DAA format (Diamond alignment format), including filtering of dominated alignments. Taxonomic and functional binning of the reads or contigs is then performed using the Meganizer program and the results are appended to the DAA file. The meganized DAA file can then opened and interactively analyzed in MEGAN-LR

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