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Fig. 3 | Biology Direct

Fig. 3

From: ASXL gain-of-function truncation mutants: defective and dysregulated forms of a natural ribosomal frameshifting product?

Fig. 3

Synonymous site conservation in the ASXL1 and ASXL2 coding regions in tetrapods. a In each subfigure, the top panel shows a schematic of the zero-frame ORF (pale blue) and the overlapping TF ORF (pink). The next two panels show positions of stop codons (blue) in the +1 and +2 reading frames, and alignment gaps (grey) in each sequence of the sequence alignment. The vertical green line in the +1 frame panel shows the position of the putative frameshift site. The bottom two panels show the synonymous site conservation analysis, with the magenta line (lower panel) indicating the ratio of the observed number of substitutions within a given window to the number expected under a null model of neutral evolution at synonymous sites, and the red line (upper panel) showing the corresponding p-value. The analysis uses a 25-codon sliding window. The horizontal dashed grey line indicates a p = 0.05 threshold after a correction for multiple testing (namely scaling by [25-codon window size]/[ASXL CDS length]. b As above, but with a 9-codon sliding window. The vertical green and blue lines show the positions of the putative frameshift site and the conserved EH[N/S]Y, respectively. (See Additional file 1: Figs. S2 and S3 for teleost fish besides separate mammalian, sauropsid and amphibian analyses)

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