Table 1 The four genes analyzed in this study
| Gene | # of residues | Deep mutational scanning | Alignment details |
|---|---|---|---|
| Yeast Gal4 DNA binding domain | 64 | [31] | 17 sequences with 87% and 59% avg and min pairwise protein identity |
| β-lactamase | 263 | [14] | 85 sequences with 82% and 63% avg and min pairwise protein identity |
| Influenza nucleoprotein (NP) | 498 | [32] | 180 sequences with 95% and 90% avg and min pairwise protein identity |
| Influenza H1 hemagglutinin (HA) | 564 | [33] | 111 sequences with 95% and 87% avg and min pairwise protein identity |