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Fig. 1 | Biology Direct

Fig. 1

From: Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells

Fig. 1

Insertion of a chloramphenicol resistance gene decreases the number of fluorescent cells measured in a GFxFP assay. a Schematic representation of the interrupted GFP cassette, depicted as on Additional file 1, with a chloramphenicol resistance gene (middle dark grey box) inserted between the homologous sequences of the GFP halves b The percentages of GFP positive cells are compared when using the reporter assay with either the original (EGxxFP, green bars) or the additional chloramphenicol resistance gene containing (GF-chl-FP, purple bars) plasmids with the interrupted GFP-halves. In each case, two targets were tested, PrP10 and Sp1, using SpCas9 nuclease and the corresponding gRNAs along with the reporter plasmids in N2a cells. As controls, inactive SpCas9 was used in both cases (red bars, dSpCas9). Values are normalized to the transfection efficiencies measured by using the fluorescence of iRFP670 used as transfection control. Error bars show the mean ± standard deviation of percentages measured in N = 3 independent transfections. The insertion of the chloramphenicol cassette decreased the number of the fluorescent cells in both the active- and dead SpCas9 containing samples

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