TY - JOUR AU - Tóth, Eszter AU - Weinhardt, Nóra AU - Bencsura, Petra AU - Huszár, Krisztina AU - Kulcsár, Péter I. AU - Tálas, András AU - Fodor, Elfrieda AU - Welker, Ervin PY - 2016 DA - 2016/09/14 TI - Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells JO - Biology Direct SP - 46 VL - 11 IS - 1 AB - Cpf1 nucleases have recently been repurposed for site-specific genome modification. Two members of the Cpf1 family, the AsCpf1 from Acidaminococcus sp. and the LbCpf1 from Lachnospiraceae bacterium were shown to induce higher indel frequencies than SpCas9 when examining four randomly-selected target sequences for each type of nuclease. Whether they are a real match for Cas9 nucleases, however, remains to be verified. SN - 1745-6150 UR - https://doi.org/10.1186/s13062-016-0147-0 DO - 10.1186/s13062-016-0147-0 ID - Tóth2016 ER -