Fig. 5

Expected reproducibility given experiment depth and coverage. a Per-position correlations between profiles (median per experimental replicate pair) against the combined replicates’ depth (reads per nucleotide position). Regression line (orange) shows the linear regression between correlation and log-depth, with the 95 % confidence intervals of the model parameters marked within the orange area. b Same for the subset of 20 % highly expressed genes in each dataset. c Here correlations and depth were computed for individual genes and binned into 100 sets of genes with similar depth. Each line denotes the mean of the bin and the 95 % confidence interval around the mean. The 26 replicate pairs were independently colored and their genes show consistent behavior according to similar model parameters (replicates from the same experiment/organism may have similar shades). d Same as (a) for correlation versus coverage. e Same as (b) for correlation versus coverage. f Same as (c) for correlation versus coverage