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Fig. 3 | Biology Direct

Fig. 3

From: Biochemical and biophysical characterization of cell-free synthesized Rift Valley fever virus nucleoprotein capsids enables in vitro screening to identify novel antivirals

Fig. 3

HOFS are both preferentially captured and detected in the ELISA sandwich assay. a RVFV NPs derived from the top of the sucrose gradient and the bottom of the glycerol gradient were preferentially detected by the anti-RVFV NP monoclonal antibody. b Diagram of the CFPS in combination with the plate assay screening setup. I. The plates are initially coated with first Ab. II. Equal amounts of CFPS RVFV NP are loaded onto each well. III. The plates are washed with PBS containing 1 % Triton X-100. IV. The fluorescently labeled anti-RVFV NP monoclonal antibody detect only the RVFV NP oligomers

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