BrdU+ CD8 + T cells (%) |
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Experimental Group | Mouse # | Mean | SE |
p
|
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| 1 | 2 | 3 | 4 | | | |
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EGFP+ MOCK | 20.0 | 13.5 | 10.7 | 30.6 | 18.7 | 6.6 | 0.01 |
EGFP+ D1 4-5 | 32.8 | 16.3 | 18.8 | 41.7 | 27.4 | 9.9 | |
- LSKs were purified from 5FU-treated CD45.1 and CD45.2 wild type mice. CD45.1 LSK were transduced with a D1 4–5 coding retrovirus whereas CD45.2 LSK were transduced with the same control (Mock) virus. Equal numbers of each transduced LSK were injected simultaneously into lethally irradiated Rag− mice. 3 weeks after cell transfer, the proliferation rates of the EGFP+ CD8+T subset were evaluated after 3 i.p. pulses of BrdU. Table 1 shows the % of BrdU+ CD8+T cells generated from transduced LSKs (EGFP+) Mock (CD45.2) and D1 4–5 (CD45.1) in each individual mouse (n = 4). Differences in proliferation between Mock and D1 4–5 transduced lymphocytes were evaluated by the paired Student t-test