Fig. 6

Effects of cyclin D1 RNAi in LSK differentiation. Bone marrow LSK (Lin⁻Sca1⁺ ckit^high) progenitors were sorted from CD45.2+ mice BM, and were transduced with either a cyclin D1 or a control shRNA-LV-EGFP. a They were directly injected into lethally irradiated (1,700 rad) CD45.1⁺Rag⁻ mice. Results show EGFP⁺ cells in four different individual mice two months after LSK transfer. Upper graphs: EGFP⁺ thymocytes; Lower graphs: B cell lineage profiles in the BM, analyzed as described in Fig. 2. b They were cultured in in vitro conditions promoting T cell (upper graphs) or B/myeloid cell differentiation (lower graphs). Results show the phenotype of EGFP⁺ at different time points after culture