Fig. 4

Type 2 functional divergence in Bacteria 1 vs. Eukaryote. (a) Thirty four divergent sites at posterior ratio (R) 10.35 have been observed from FDII analysis of prokaryote I vs. eukaryote clusters. The putative divergent sites are marked as a highest peak in figure. The twenty five sites are observed on large subunit and nine are on the small subunit. (b) The type 2 amino acids sites are identified by DIVERGE 3.0 mapped on GGT structure (cyan color) of E. coli (2DBX). The identified FDII sites are shown in stick (magenta) whereas the inbuilt glutamate substrate is shown in green stick. (c) The FDII sites identified in Bacteria 1 vs. eukaryotes cluster comparisons are mapped on glutamate (violet) binding cavity (cyan color) of E. coli GGT within the 6 Å radius. The putative 3 divergent sites (red color) of functional divergence type 2 analyses are observed in binding cavity region of E. coli GGT