Table 2 Problems detected by different control probe-sets and their possible reasonsa
From: Microarray experiments and factors which affect their reliability
Housekeeping genes | Poly-A spike | Bacterial spike | Possible reason |
|---|---|---|---|
error | ok | ok | Poor quality of the mRNA analyzed |
error | error | ok | Problems during amplification/labeling |
error | error | error | Problems during hybridization/washing |
ok | ok | error | Inaccurate preparation of bacterial spike |
ok | error | ok | Inaccurate preparation of bacterial poly-A spike |