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Fig. 2 | Biology Direct

Fig. 2

From: Microarray experiments and factors which affect their reliability

Fig. 2

Individual steps of a microarray experiment. After isolation of sample mRNA (1) synthesis of cDNA (complementary DNA, step) chains begins with addition of oligo(dT) primers (2), then cDNA is amplified, producing cRNA (complementary RNA), which is labelled with biotin (3) and later fragmented (4). After such preparation sample cRNA is ready for hybridization with microarray probes (5) and ready for the final staining process (6)

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