Mitochondrial activity in gametes and transmission of viable mtDNA

Biology Direct

Table 1 Detection of mitochondrial membrane potential (Δψm) in animal oocytes

Animal	Δψm-sensitive mitochondrial staining	Cell type and stage (st)	Bb	Ref.
Fruit fly	MitoTracker Red CMX Ros (Molecular Probes) (M-7512)	nurse cell ec-st 6		Figure 2B in [105]
Zebrafish	MitoTracker Red 580 (Molecular Probes) (M-22425)	oocyte st 1	x	Figure 2G in [106]
	MitoTracker Red CMXRos (M-7512)	oocyte st 1	x	Figures 1, 2, 3 and 4 in [42]
	JC-1* (Cell Technology Inc.)	oocyte st 1	x	Figures 1, 2, 3 and 4 in [42]
African clawed frog	MitoTracker Red	oocyte st 2	x	Figure 1 in [40]
	JC-1	oocyte st 2	x	Figure 1 in [40]
Mouse	MitoTracker Orange (Invitrogen Ltd.)	oocyte st GV / GVBD		Figures 2, 3, 4, 5 and 6 in [107]
Mouse	JC-1 (Molecular Probes)	oocyte st MII		Figures 1,2 in [41]
Dog	MitoTracker Red CMX Ros (Molecular Probes) (M-7512)	oocyte st non-m / Ov / IVM		Figure 1 in [108]
Pig	JC-1 (Invitrogen)	oocyte st MII		Figure 1A in [43]
Dromedary camel	MitoTracker Orange CMTMRos (Molecular Probes) (M-7510)	oocyte st GV / MI / MII		Figures 2,3 in [66]
Human	JC-1 (Molecular Probes)	oocyte st GV / MI / MII		Figs 1, 2 and 3 in [57]

NOTE - Bb: Balbiani body staining/localization; ec: egg chamber; GV: immature oocyte, in which the mitochondria are aggregated around the germinal vesicle; GVBD: once oocyte maturation commences the GV breaks down and the mitochondria are seen to disperse throughout the cytoplasm during maturation, resulting in mitochondria occupying most of the egg volume; MII: metaphase II; Ov: ovulated = in vivo maturation; non-m: non-matured; IVM: in vitro maturation; *5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolylcarbocyanine iodide.

ISSN: 1745-6150