Quality control of the transcriptomic data. (A) Comparison of the two biological replicates for each subtype. Gene expression levels as size-factor adjusted, length-normalised counts are plotted for each pair of replicates as two-dimensional kernel density estimates in yellow–green with individual points plotted in light grey. Correlation coefficients are consistently high, ranging from 0.81 to 0.98. (B) Expression of splenic cell population markers. Expression levels of markers indicative of CD4+ T cells (top row) and those suggestive of other contaminant splenic cell populations (middle and bottom rows).