Alternative splicing of the gene encoding CD45 (
). (A) The first exons of the two Ptprc isoforms (Ptprc-201, Ptprc-202) annotated in the ENSEMBL GRCm38v70 mouse genome. Exons 4–6 (highlighted by grey box) are skipped in Ptprc-202 leading to a shorter transcript that is preferentially expressed following T cell activation. (B) Relative expression of Ptprc splice isoforms in the CD4+ subtypes was estimated using the mixture-of-isoforms (MISO) model to calculate percent spliced in (PSI) values for each isoform in each subtype.