Figure 3

Output page of Off-Spotter. As seen in the figure, the user can sort the results by all the information presented in the columns and also by multiple columns at the same time, can show/hide columns, download the results, select the number of results presented per page and navigate through the pages easily. The mismatches are indicated by red lowercase letters for each off-target. The annotation reports include the ENSEMBL gene identifiers, transcript identifiers, and common gene names. Two types of tables are available per search. The top table provides the summary of the results, i.e. the number of results per gRNA found in the input, the strand on which it was found in those instances where a target sequence was entered, and a histogram of the number of off-targets as a function of the number of mismatches. The table for each individual gRNA includes all off-targets for the corresponding gRNA together with detailed genomic location information and genomic annotation. See also text.